Dr. Pablo Sobrado of the Chemistry Department at Missouri S&T will make a presenation titled "Hydroxamate containing siderophores biosynthesis and inhibition".

Flavin-dependent enzymes are versatile catalysts involved in microbial virulence, bioremediation, and plant metabolism, among other functions. Our research group has made major contributions to elucidating the mechanism of action of several flavin-dependent enzymes in these areas. This presentation will focus on structural and mechanistic studies of SidA, a flavin-dependent monooxygenase essential for the biosynthesis of hydroxamate-containing siderophores in the pathogenic fungus Aspergillus fumigatus. Because siderophores are required for iron acquisition and thus growth during infection, SidA has been identified as a potential drug target. The data will highlight the identification of flavin and protein motions crucial for substrate binding and release. In addition, the identification and validation of SidA inhibitors using a high-throughput assay will also be described